Proton translocation in cytochrome c oxidase: Insights from proton exchange kinetics and vibrational spectroscopy
نویسندگان
چکیده
منابع مشابه
Oxygen Reduction and Proton Translocation by Cytochrome C Oxidase
.............................................................................................................5 ABBREVIATIONS..................................................................................................6 LIST OF ORIGINAL PUBLICATIONS...............................................................7
متن کاملKinetics of proton pumping in cytochrome c oxidase.
We propose a simple model of cytochrome c oxidase, including four redox centers and four protonable sites, to study the time evolution of electrostatically coupled electron and proton transfers initiated by the injection of a single electron into the enzyme. We derive a system of master equations for electron and proton state probabilities and show that an efficient pumping of protons across th...
متن کاملNew insights on the cytochrome c oxidase proton pump.
Cytochrome c oxidase vesicles were used to show that, under appropriate experimental conditions: (1) no net deprotonation of the vesicular membrane or of the incorporated enzyme occurs during the oxidation of ferrocytochrome c; (2) the pH equilibration kinetics of a respiration-induced pH gradient across the bilayer are a simple function of the ohmic proton-conductance properties of the membran...
متن کاملProton-pumping mechanism of cytochrome C oxidase.
Cytochrome c oxidase (CcO), as the terminal oxidase of cellular respiration, coupled with a proton-pumping process, reduces molecular oxygen (O(2)) to water. This intriguing and highly organized chemical process represents one of the most critical aspects of cellular respiration. It employs transition metals (Fe and Cu) at the O(2) reduction site and has been considered one of the most challeng...
متن کاملProton pumping by cytochrome c oxidase.
Proton Pumping in Cotychrome c Oxidase by Jianxun Lu Advisor: Professor Marilyn Gunner Cytochrome c oxidase (CcO) is a large trans-membrane protein, which is the final enzyme in the respiratory electron transport chain in mitochondria or aerobic bacteria. It implements proton pumping through the mitochondrial membrane against the electrochemical gradient, by utilizing the chemical energy releas...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2015
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2014.09.008